骨桥蛋白在儿童中枢神经系统白血病中的表达及髓外浸润机制的研究(1)
[摘要]目的 探讨骨桥蛋白(OPN)在儿童中枢神经系统白血病(CNSL)中的表达以及髓外浸润的机制。方法 选取2016年1月～2018年1月我院收治的10例CNSL患儿、20例非CNSL急性白血病患兒以及20例非肿瘤疾病患儿，分别标记为观察组、对照组及常规组。通过ELISA检测三组患儿治疗前以及观察组与对照组患儿完全缓解后血清中的OPN含量，并通过RT-PCR检测三组患儿治疗前以及观察组与对照组患儿完全缓解后的骨髓OPN表达情况。将培养的白血病Jurkat细胞分为空转组与转染组，其中空转组不做任何处理，转染组将合成OPN特异性的siRNA转染到Jurkat细胞中。对比分析培养的白血病Jurkat细胞空转组与转染组的OPN表达情况、细胞存活率以及细胞侵袭力。结果 治疗前，观察组患儿的血清OPN含量及骨髓OPN表达均高于对照组与常规组，差异有统计学意义(P<0.05);对照组患儿的血清OPN含量及骨髓OPN表达均高于常规组，差异有统计学意义(P<0.05)。完全缓解后，观察组与对照组患儿的血清OPN含量及骨髓OPN表达均低于治疗前，差异有统计学意义(P<0.05);完全缓解后，观察组患儿的血清OPN含量及骨髓OPN表达高于对照组，差异有统计学意义(P<0.05)。转染组白血病Jurkat细胞的OPN表达、细胞存活率以及下层浸润细胞数与总细胞数比率均低于空转组，差异有统计学意义(P<0.05)。结论 OPN在儿童CNSL患儿的表达中高于非CNSL急性白血病及其他肿瘤疾病患儿，并且其在白血病细胞浸润中枢神经系统中起着一定的作用。
[关键词]中枢神经系统白血病;骨桥蛋白;急性白血病;ELISA检测
[中图分类号] R543.5 [文献标识码] A [文章编号] 1674-4721(2019)11(a)-0008-05
Study of osteopontin expression in childhood central nervous system leukemia and its mechanism of extramedullary infiltration
WANG Chun-jing FANG Xi-min CHEN Sen-min LIU Shi-lin
Department of Hematology and Oncology， Shenzhen Children′s Hospital， Guangdong Province， Shenzhen 510038， China
[Abstract] Objective To investigate the expression of osteopontin (OPN) in children′s central nervous system leukemia (CNSL) and the mechanism of extramedullary infiltration. Methods From January 2016 to January 2018， 10 children with CNSL， 20 children with non-CNSL acute leukemia and 20 children with non-neoplastic diseases treated in our hospital were selected and labeled as observation group， control group and routine group respectively. The levels of OPN in serum of children in three groups before treatment and after complete remission in observation group and control group were detected by ELISA. The expression of OPN in bone marrow of children in three groups before treatment and after complete remission in observation group and control group was detected by RT-PCR. Cultured Jurkat cells were divided into blank group and transfection group， in which the blank group did not do any treatment， and the transfection group transfected the synthetic OPN-specific siRNA into Jurkat cells. The expression of OPN， cell viability and invasiveness of cultured Jurkat cells were compared between the blank group and the transfected group. Results Before treatment， the serum OPN content and bone marrow OPN expression in the observation group were higher than those in the control group and the routine group， and the differences were statistically significant (P<0.05). The level of serum OPN and the expression of bone marrow OPN in the control group were higher than those in the routine group， and the differences were statistically significant (P<0.05). After complete remission， the serum OPN content and bone marrow OPN expression in the observation group and the control group were lower than those before treatment， and the differences were statistically significant (P<0.05). After complete remission， the serum OPN content and bone marrow OPN expression in the observation group were higher than those in the control group， with statistical significance (P<0.05). The expression of OPN， cell viability and ratio of infiltrating cells to total cells in the lower layer in the transfected group were lower than those in the blank group， with statistical significance (P<0.05). Conclusion The expression of OPN in children with CNSL is higher than that in non-CNSL children with acute leukemia and other cancer diseases， and it plays a certain role in leukemia cell infiltration into the central nervous system., http://www.100md.com(王春静 方希敏 陈森敏 刘仕林)
[关键词]中枢神经系统白血病;骨桥蛋白;急性白血病;ELISA检测
[中图分类号] R543.5 [文献标识码] A [文章编号] 1674-4721(2019)11(a)-0008-05
Study of osteopontin expression in childhood central nervous system leukemia and its mechanism of extramedullary infiltration
WANG Chun-jing FANG Xi-min CHEN Sen-min LIU Shi-lin
Department of Hematology and Oncology， Shenzhen Children′s Hospital， Guangdong Province， Shenzhen 510038， China
[Abstract] Objective To investigate the expression of osteopontin (OPN) in children′s central nervous system leukemia (CNSL) and the mechanism of extramedullary infiltration. Methods From January 2016 to January 2018， 10 children with CNSL， 20 children with non-CNSL acute leukemia and 20 children with non-neoplastic diseases treated in our hospital were selected and labeled as observation group， control group and routine group respectively. The levels of OPN in serum of children in three groups before treatment and after complete remission in observation group and control group were detected by ELISA. The expression of OPN in bone marrow of children in three groups before treatment and after complete remission in observation group and control group was detected by RT-PCR. Cultured Jurkat cells were divided into blank group and transfection group， in which the blank group did not do any treatment， and the transfection group transfected the synthetic OPN-specific siRNA into Jurkat cells. The expression of OPN， cell viability and invasiveness of cultured Jurkat cells were compared between the blank group and the transfected group. Results Before treatment， the serum OPN content and bone marrow OPN expression in the observation group were higher than those in the control group and the routine group， and the differences were statistically significant (P<0.05). The level of serum OPN and the expression of bone marrow OPN in the control group were higher than those in the routine group， and the differences were statistically significant (P<0.05). After complete remission， the serum OPN content and bone marrow OPN expression in the observation group and the control group were lower than those before treatment， and the differences were statistically significant (P<0.05). After complete remission， the serum OPN content and bone marrow OPN expression in the observation group were higher than those in the control group， with statistical significance (P<0.05). The expression of OPN， cell viability and ratio of infiltrating cells to total cells in the lower layer in the transfected group were lower than those in the blank group， with statistical significance (P<0.05). Conclusion The expression of OPN in children with CNSL is higher than that in non-CNSL children with acute leukemia and other cancer diseases， and it plays a certain role in leukemia cell infiltration into the central nervous system., http://www.100md.com(王春静 方希敏 陈森敏 刘仕林)